Biarritz Olympique qualified as Pool 1 winners after making home advantage count against defending champions London Wasps with a 18-15 victory while Leinster claimed the No 1 ranking spot with the tournament's only 100 per cent Pool record after beating Benetton Treviso 57-17 at Lansdowne Road.
Toulouse qualified on Friday night after topping Pool 3 with 24 points and a guaranteed home quarter-final.
The winners from Pool 5 and Pool 6 will be decided tomorrow (Sunday). Who will join Northampton Saints as the best runners-up from all six groups will also be determined.
Heineken Cup quarter-final qualifiers:
Pool 1 Winners - Biarritz Olympique 22 points (20 tries)
Pool 2 Winners - Leinster 26 points (33 tries)
Pool 3 Winners - Toulouse 24 points (21 tries); Runners-up - Northampton Saints 21 points (11 tries)
Pool 4 Winners - Munster - 22 points (12 tries)
Pool 5 Winners - To Be Decided Sunday
Pool 6 Winners - To Be Decided Sunday