Inspirational club captain Rougerie, back rower Vosloo and front row forward Kotze remain on individual programmes and a statement on the club website declares their chances of being fit remain uncertain.
They will have make-or-break tests on Thursday but in the meantime head coach Vern Cotter and his staff must prepare without them.
But there was better news for the club bidding to make history by winning the trophy with an unprecedented nine straight victories.
Lock Jamie Cudmore (shoulder), wing Naipolioni Nalaga (ankle) and prop Vincent Debaty (thigh) were all able to take part in the first squad training session of the week.
They will all be in the mix when Cotter settles on his 23-player squad for the prestigious match but for Rougerie & Co it is a desperate race against time.