The 41-31 triumph in the west country kept Biarritz on course to try to become the first team to successful retain the title. As for Gloucester, the rest fo their season will be dedicated to qualifying for the Heineken Cup via the Aviva Premiership.
Director of rugby Nigel Davies was full of praise for the streetwise Biarritz side, who outscored his team by five tries to three.
"We came off the back of a big performance last weekend and we were hoping to back that up with another big performance, but we didn't have the same intensity. That's why we couldn't play our rugby until the last few minutes," said Davies..
"Maybe they played smarter rugby than us. We had two yellow cards - the first was a joke and the second was justified. We wanted to bring more energy to the game, but they slowed it down and they have some smart players.
"We are a team at Gloucester that is evolving and developing and maybe we played too much rugby, but I am not going to stop them from playing."