Andy Robinson's side are still looking for their first victory in this year's tournament and face an Italian side full of confidence after defeating defending Grand Slam Champions France last weekend.
"It's a massive game for us, we know that," said captain Alastair Kellock.
"We've got to take positives from the England game and use the momentum we've got - even though we were beaten - to push for a top performance that it will take to beat the Italians, especially on the back of the win they've just had."
"We had a real chance of winning that game; everybody realises how special it would've been had we done so.
"We've got to look at the things we did well, take them on board and push to get a performance this week.
"Over the last few games our line-out has gone well and it didn't function anywhere near where it needed to. We've got a lot to work on."
Scotland: Paterson (Edinburgh); M Evans (Glasgow Warriors), Ansbro (Northampton), S Lamont (Scarlets), Danielli (Ulster); Jackson (Glasgow Warriors), Lawson (Gloucester); Jacobsen (Edinburgh), Ford (Edinburgh), Low (Glasgow Warriors), Gray (Glasgow Warriors), Kellock (Glasgow Warriors, captain), Hines (Leinster), Barclay (Glasgow Warriors), Brown (Saracens).
Replacements: S Lawson (Gloucester), E Murray (Newcastle), Vernon (Glasgow Warriors), Strokosch (Gloucester), Blair (Edinburgh), Parks (Cardiff Blues), De Luca (Edinburgh).