Jones is back in the fly-half jersey after missing the victory over Scotland and will win his 98th cap and Cardiff Blues wing Leigh Halfpenny has recovered from an ankle injury to be named amongst the replacements.
"We have selected the strongest possible side, showing the utmost respect to Italy," said Wales head coach Warren Gatland.
"We know only too well what tough opponents they are in Rome and so we have picked the strongest team available to us."
Wales: Lee Byrne (Ospreys); Morgan Stoddart (Scarlets), James Hook (Ospreys), Jamie Roberts (Cardiff Blues), Shane Williams (Ospreys); Stephen Jones (Scarlets), Mike Phillips (Ospreys); Paul James (Ospreys), Matthew Rees (Scarlets, capt), Craig Mitchell (Ospreys), Bradley Davies (Blues), Alun Wyn Jones (Ospreys), Dan Lydiate (Newport Gwent Dragons), Sam Warburton (Blues), Ryan Jones (Ospreys).
Replacements: Richard Hibbard (Ospreys), John Yapp (Blues), Jonathan Thomas (Ospreys), Josh Turnbull (Scarlets), Tavis Knoyle (Scarlets), Rhys Priestland (Scarlets), Leigh Halfpenny (Blues).