The Scottish side will be looking for a repeat of their 2009 triumph at the Stade Ernest-Wallon when they came away with a famous 33-26 victory.
Head coach Sean Lineen will take heart from that success but is looking for a better performance from his team than the one at Firhill on Friday.
"I'm confident well do a lot better. It's a fantastic stadium to play at, but the guys just need to focus and make sure we get the performance we need." He said.
"We can't take much confidence from the weekend because we didn't play that well.
"Our set piece wasn't good but we never gave up and that's hugely encouraging. We can't give them that much ball or that much respect this weekend.
"We just a little more leadership and experience. We had a good team out last weekend but it's now about putting our performance right in such a massive game.
"There aren't many bigger games than Toulouse away in the Heineken Cup."
Glasgow Warriors team to play Toulouse
15 Bernardo Stortoni
14 Hefin O'Hare
13 Max Evans
12 Graeme Morrison
11 DTH van der Merwe
10 Ruaridh Jackson
9 Colin Gregor
1 Jon Welsh
2 Dougie Hall
3 Moray Low
4 Richie Gray
5 Alastair Kellock CAPTAIN
6 Robert Harley
7 John Barclay
8 Richie Vernon
Substitutes: 16 Fergus Thomson, 17 Ryan Grant, 18 Kevin Tkachuk, 19 Aly Muldowney, 20 Chris Fusaro, 21 Henry Pyrgos, 22 Peter Murchie, 23 Federico Aramburu