The Welsh side produced a creditable performance in their 30-14 defeat in Toulouse - Kahn Fotualii scoring an opportunist try - but it was still a bonus point victory for the French giants and leaders of this hugely competitive Pool.
Toulouse, who lifted the trophy in 1996, 2003, 2005 and 2010, are one of the favourites this season and will want to come away with all the points on offer in Wales.
A bonus point victory would put more pressure on Pool rivals, the Leicester Tigers, who are only three point behind the French side but who travel to play Treviso in Italy.
The Ospreys have reached three Heineken Cup quarter-finals but this season's failure will mean they haven't reached the knockout stages for the last three campaigns in Europe.
Toulouse, on the other hand, will already be thinking of a 16th quarter-final and will be looking for a vital home draw in the knockout stages as they go looking for a fifth title.
• Louis Picamoles and Yoann Huget of Toulouse have beaten more defenders than anyone else in the tournament this season (14 each).
• Ospreys boast the top goal kickers in the tournament, as a team their success rate of 96% is not matched by any other side.
• Toulouse collectively average over 21 defenders beaten per match, the highest figure in the tournament.
• Ospreys have lost all three of their previous Heineken Cup meetings with Toulouse, with the closest margin being 16 points (last week's 30-14 defeat).