Buchurest snatched a late victory in Romania last week over Agen thanks to Florin Vlaicu's last-minute penalty.
That victory was the club's most significant result since beating Montauban exactly five years before and also secured a second win of the campaign. Another success would signal their best ever showing in Europe.
Agen's slim hopes of progressing were ended in Romania but are now under real pressure to produce a result for their supporters.
The former Challenge Cup finalists are in the midst of a slump having now won just one of their last eight games in all competitions after resting several leading players last week.
• Bucharest Wolves' Florin Vlaicu, the team's top points scorer in this tournament, needs six points to reach 200 points in the Amlin Challenge Cup.
• Agen have lost six of their last eight Amlin Challenge Cup matches.
• Bucharest secured back-to-back victories for the first time in their tournament history against Cammi Rugby Calvisano and Agen.
• Agen have conceded a competition low 9.5 turnovers per game so far in 2012/13.