That sparked three successive defeats in a row for the Welsh region, who saw Shane Williams, their top try scorer in the Heineken Cup, retire in the summer.
However three wins in a row have the Ospreys back on track ahead of what will be head coach Steve Tandy's first game in charge in Europe.
Last season saw Treviso enjoy their best campaign in the Heineken Cup for seven years with victory over Biarritz Olympique and a draw against the Ospreys.
Kris Burton is wracking up the points in front of goal and in Italy flanker Roberto Barbieri, Treviso have the highest scoring forward in the Pro12 with six tries already this season. The Ospreys will be banking on Dan Biggar being on song with the boot.