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Toulon were beaten finalists in the 2010 Amlin Challenge Cup final, while Biarritz were runners-up in the Heineken Cup in 2006 and 2010. Now they aim to make it third time lucky.
"This is a step closer to getting into the Heineken Cup next season. It's the number one club competition in the world and it means the world to Biarritz to play in it," said Biarritz full back Iain Balshaw.
"We're not going to qualify for it through the Top14 so we have a huge 80 minutes coming up against Toulon. Jonny Wilkinson kicked them to victory over Stade Francais and they are a fantastic side.
"That last minute drop goal was unbelievable, but that why he is one of the greatest players of all time. He has been doing that consistently since he was 18-years-old and I know what practice he puts in - he thoroughly deserves everything he gets.
"The final will also be the last game before Sylvain retires. He has had a fantastic career, especially given the position he plays, and we would love to send him out on a high note."
