Harlequins and London Wasps go head-to-head at the Twickenham Stoop on Friday night for a place in the Amlin Challenge Cup three weeks later with Harlequins enjoying the advantage of playing at home as they bid to go a giant step closer to a third tournament title.
The 2001 and 2004 tournament winners drew 29-29 at Wasps at the start of the season and then won17-10 in the home return Premiership encounter but head coach John Kingston accepts this time it is sudden death - and that the Amlin Challenge Cup is now even tougher to win with the three Heineken Cup runners-up joining the five Amlin Pool winners.
"The Amlin Challenge Cup is even more competitive than it's ever been," said Kingston. "When you look at the quality of the teams involved it is far from a subsidiary competition.
"It's a very big competition and there are some very big sides left in it. We're looking forward to the quarter-final having earned the right to be there after coming through a difficult group.
"Wasps are a very stubborn group and work hard for each other. They have had their ups and downs over recent weeks but what you know about Wasps is that they always give you a tough match.
"They are very physical and Harlequins versus Wasps matches are no different.
"We want to make sure we capitalise on home advantage and nail the win on Friday night. When our fans turn up in their numbers and they get behind the team it makes a massive difference. I hope we'll get a decent crowd in because it can make a huge difference.
"We are well aware that it's knock-out rugby on Friday night. We have just come off the back of a massive sell-out game on Saturday against Leicester and we haven't had much time to think about it but the reality is that we're playing in a European quarter-final."
Harlequins went down 17-13 to Heineken Cup quarter-finalists Leicester Tigers but Kingston believes his side can bounce straight back.
"We are frustrated because we feel we did well enough to win the game against Leicester," he said. "We had opportunities to have put the game out of sight but a series of circumstances, some of which were in our control and some of which weren't, took the game away from us.
"We are not happy whenever we get turned over, particularly on our own patch, which doesn't happen very often.
"So we have obviously got to look to put that right. Our response to losing the LV= semi-final was pretty dramatic in the way that we then put 50 points on Gloucester and we are looking for a similar response on Friday night.
"However, I am sure they're still hurting from the Abu Dhabi game and they will be looking to try and get something back over us. It's up to us to impose our game on them on our own patch.
"And when you get key players back, it makes a difference. We feel that we've been playing quite well and that's where you get your confidence from - it's not because 'oh, we beat this lot two months ago'.
"That's not the way it works. I've got the utmost respect for the way Wasps conduct themselves and the physicality that they play the game with and we're wary of the threat they pose."