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The squad will meet at St Andrews next week to begin preparations for their first game of the championships, away to reigning champions France on Saturday February 5.
"Selection reflects current form and the performances of players during our matches in the autumn," said Robinson.
"There's always excitement in the air at this time of year as we look forward to the RBS 6 Nations Championship.
"I've been impressed by Robert Harley since I first saw him play in a pro-team back-up game some seasons back.
"He has performed consistently for Glasgow this season in a position where we have strength and there is considerable competition for a starting place.
"Jon Welsh is another who has benefited from raising his game in the face of intense competition and has a good work ethic.
"Jack Cuthbert has had a really good couple of months having won his place in the Bath first XV. He was also on duty during the Nations Cup in Romania last summer and I've said often enough that players who perform for Scotland A are very much in the frame."
Injury has ruled out Glasgow's Chris Cusiter and Graeme Morrison, Gloucester's Alasdair Dickinson and Jim Hamilton and Edinburgh's Phil Godman.
Scotland squad: Backs - Joe Ansbro (Northampton Saints), Mike Blair (Edinburgh), Jack Cuthbert (Bath), Simon Danielli (Ulster), Nick De Luca (Edinburgh), Max Evans (Glasgow Warriors), Alex Grove (Worcester Warriors), Ruaridh Jackson (Glasgow Warriors), Greig Laidlaw (Edinburgh), Rory Lamont (Toulon), Sean Lamont (Scarlets), Rory Lawson (Gloucester), Dan Parks (Cardiff Blues), Chris Paterson (Edinburgh), Hugo Southwell (Stade Francais) and Nikki Walker (Ospreys).
Forwards - John Barclay, Johnnie Beattie (both Glasgow Warriors), Kelly Brown (Saracens), Geoff Cross, Ross Ford (both Edinburgh), Richie Gray, Dougie Hall, Robert Harley (all Glasgow Warriors), Nathan Hines (Leinster), Allan Jacobsen (Edinburgh), Alastair Kellock (Glasgow Warriors), Scott Lawson (Gloucester), Moray Low (Glasgow Warriors), Scott MacLeod (Edinburgh), Euan Murray (Newcastle Falcons), Ross Rennie (Edinburgh) and Richie Vernon and Jon Welsh (both Glasgow Warriors).
