The Ospreys will still be reeling from their failure to make the last eight of the Heineken Cup after their 15-15 draw to the Leicester Tigers was not enough to keep them in the tournament.
The Welsh side's elimination means there is no Welsh representation in the knock-out stages of the Heineken Cup for the second time in the last three seasons but the reigning Pro12 champions will be determined to sign off in style.
Wing Eli Walker, who set up the final Ospreys try for replacement centre Jonathan Spratt, was rewarded for his devastating display with call-up to the Wales 6 Nations squad.
The Ospreys travel to face fellow Pro12 side Benetton Treviso with both sides only having pride to play for in the final game of the Pool at Stadio Comunale di Monigo.
The Welsh side recorded a 38-17 victory over the Italians in the Pool opener at the Liberty Stadium but the Ospreys know all about the threat posed by Treviso at home.
The Italians held the Ospreys 26-26 on their own patch in last season's tournament and are still looking for their first victory in the Pool and will be determined to finish on a high.
Match Facts
- Treviso have won just one previous Heineken Cup Round 6 match - 33-19 at home to Pontypridd in 1998/99.
- The Italians have lost their last eight Heineken Cup ties since beating Biarritz Olympique 30-26 at Stadio Monigo on 10 December 2011.
- Ospreys' 36-5 defeat at Biarritz at this stage last year ended a sequence of five successive Round 6 wins for the Welsh region.
- Ospreys are without a win in their last 10 away games in Europe since beating Viadana 62-7 at Reggio nell'Emilia in December 2009.
- Treviso have been penalised more than any other club in the Heineken Cup this season, averaging 18 in their five matches to date.
(3)